Bioorthogonal Reaction

* ADIBO, N₃, Propyne, Maleimide

* Specific markers of target molecules in living cells

* Application: Protein-small molecule conjugation;

                       Protein-peptide conjugation

Heterobifunctional PEG Linkers

* Most notable and useful in protein crosslinking

* In a controlled, two-step reaction

* Avoid dimers and polymers

* R₁/R₂ = HS, COOH, NH₂, OH, Hydrazide, etc.

* PEG repeat units up to 48

Linkers with Protecting Groups

* Used for step by step linking, peptide modification

* Fmoc, Boc, ^tBu, CBZ, SPDP Protected PEG

* PEG repeat units up to 36

Homobifunctional PEG Linkers

* Have advantages in one-step crosslinking 

   reactions

* Ideal strategy for locking two proteins

* R₁ = OH, NH₂, COOH, NHS, Mal, Propyne,

   Hydrazide, etc.

* PEG repeat units up to 48

PEGylation Reagents

* Increase solubility, prolong stability,

  reduce immunogenicity

* Alter properties of proteins, immunogens, etc

* R₁ = OH, NH₂, COOH, NHS, SH, Mal, N₃,

   Propyne, etc.

* PEG repeat units up to 48

Biotinylation Reagents

* Various end groups available

* won't affact the biological activity of protein

* Bio-labeling and Diagostics

Hydrophobic Linkers

* Introduce maleimide to amine reactive molecules

* Hydrophobic spacers

Alkanthiols for Self-Assembled Monolayers

* m = 6~12, n = 1~24

* Surface Reaction

* Specific binding receptors

Polydisperse PEG Derivatives

* Average M.W. = 1000~5000

* NH₂, COOH, NHS, Mal, SH, N₃, Propyne, Biotin, etc.

* Various combination of end groups